Abstract
A method is described for identifying the afferents to confined areas within the central nervous system using iontophoretic application of the fluorescent tracer, Fluoro-Gold (FG). Unlike other fluorescent tracers, it is possible to make focal iontophoretic injections through small-tipped micropipettes, and electrophysiological recordings from the injection pipette can be used to define structures prior to injections. Retrograde labeling with FG appears to be as sensitive as wheatgerm agglutinin-conjugated horseradish peroxidase visualized with tetramethylbenzidine. Furthermore, iontophoretically applied FG does not appear to be taken up and transported retrogradely by fibers of passage. Finally, retrograde transport of FG can be combined with immunofluorescence without: appreciable loss of sensitivity in either label.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 259-271 |
| Number of pages | 13 |
| Journal | Brain Research |
| Volume | 475 |
| Issue number | 2 |
| DOIs | |
| State | Published - Dec 20 1988 |
| Externally published | Yes |
ASJC Scopus subject areas
- Neuroscience(all)
- Molecular Biology
- Clinical Neurology
- Developmental Biology
Keywords
- Afferent pathway
- Fluorescent tracer
- Fluoro-Gold
- Immunofluorescent
- Locus coeruleus
- Nucleus basalis
- Raphe
- Rat
- Retrograde tracing
- Substantia nigra