The iontophoretic application of Fluoro-Gold for the study of afferents to deep brain nuclei

Vincent A. Pieribone, Gary Aston-Jones

Research output: Contribution to journalArticlepeer-review

97 Scopus citations

Abstract

A method is described for identifying the afferents to confined areas within the central nervous system using iontophoretic application of the fluorescent tracer, Fluoro-Gold (FG). Unlike other fluorescent tracers, it is possible to make focal iontophoretic injections through small-tipped micropipettes, and electrophysiological recordings from the injection pipette can be used to define structures prior to injections. Retrograde labeling with FG appears to be as sensitive as wheatgerm agglutinin-conjugated horseradish peroxidase visualized with tetramethylbenzidine. Furthermore, iontophoretically applied FG does not appear to be taken up and transported retrogradely by fibers of passage. Finally, retrograde transport of FG can be combined with immunofluorescence without: appreciable loss of sensitivity in either label.

Original languageEnglish (US)
Pages (from-to)259-271
Number of pages13
JournalBrain Research
Volume475
Issue number2
DOIs
StatePublished - Dec 20 1988
Externally publishedYes

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

Keywords

  • Afferent pathway
  • Fluorescent tracer
  • Fluoro-Gold
  • Immunofluorescent
  • Locus coeruleus
  • Nucleus basalis
  • Raphe
  • Rat
  • Retrograde tracing
  • Substantia nigra

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