The machinery of vesicle fusion

Abigail E. Stanton; Frederick M. Hughson

doi:https://doi.org/10.1016/j.ceb.2023.102191

The machinery of vesicle fusion

Abigail E. Stanton, Frederick M. Hughson

Princeton University

Research output: Contribution to journal › Review article › peer-review

Abstract

The compartmentalization of eukaryotic cells is reliant on the fidelity of vesicle-mediated intracellular transport. Vesicles deliver their cargo via membrane fusion, a process requiring membrane tethers, Sec1/Munc18 (SM) proteins, and SNAREs. These components function in concert to ensure that membrane fusion is efficient and accurate, but the mechanisms underlying their cooperative action are still in many respects mysterious. In this brief review, we highlight recent progress toward a more integrative understanding of the vesicle fusion machinery. We focus particular attention on cryo-electron microscopy structures of intact multisubunit tethers in complex with SNAREs or SM proteins, as well as a structure of an SM protein bound to multiple SNAREs. The insights gained from this work emphasize the advantages of studying the fusion machinery intact and in context.

Original language	English (US)
Article number	102191
Journal	Current Opinion in Cell Biology
Volume	83
DOIs	https://doi.org/10.1016/j.ceb.2023.102191
State	Published - Aug 2023

ASJC Scopus subject areas

Cell Biology

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https://doi.org/10.1016/j.ceb.2023.102191

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title = "The machinery of vesicle fusion",

abstract = "The compartmentalization of eukaryotic cells is reliant on the fidelity of vesicle-mediated intracellular transport. Vesicles deliver their cargo via membrane fusion, a process requiring membrane tethers, Sec1/Munc18 (SM) proteins, and SNAREs. These components function in concert to ensure that membrane fusion is efficient and accurate, but the mechanisms underlying their cooperative action are still in many respects mysterious. In this brief review, we highlight recent progress toward a more integrative understanding of the vesicle fusion machinery. We focus particular attention on cryo-electron microscopy structures of intact multisubunit tethers in complex with SNAREs or SM proteins, as well as a structure of an SM protein bound to multiple SNAREs. The insights gained from this work emphasize the advantages of studying the fusion machinery intact and in context.",

author = "Stanton, {Abigail E.} and Hughson, {Frederick M.}",

note = "Funding Information: We thank Mary Munson, Jose Rizo, Bill Wickner, Yongli Zhang, and members of the Hughson laboratory past and present for helpful advice and discussion. This work was supported by National Institutes of Health grants T32GM007388 (AES) and R01GM071574 (FMH). Publisher Copyright: {\textcopyright} 2023 Elsevier Ltd",

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language = "English (US)",

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journal = "Current Opinion in Cell Biology",

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T1 - The machinery of vesicle fusion

AU - Stanton, Abigail E.

AU - Hughson, Frederick M.

N1 - Funding Information: We thank Mary Munson, Jose Rizo, Bill Wickner, Yongli Zhang, and members of the Hughson laboratory past and present for helpful advice and discussion. This work was supported by National Institutes of Health grants T32GM007388 (AES) and R01GM071574 (FMH). Publisher Copyright: © 2023 Elsevier Ltd

PY - 2023/8

Y1 - 2023/8

N2 - The compartmentalization of eukaryotic cells is reliant on the fidelity of vesicle-mediated intracellular transport. Vesicles deliver their cargo via membrane fusion, a process requiring membrane tethers, Sec1/Munc18 (SM) proteins, and SNAREs. These components function in concert to ensure that membrane fusion is efficient and accurate, but the mechanisms underlying their cooperative action are still in many respects mysterious. In this brief review, we highlight recent progress toward a more integrative understanding of the vesicle fusion machinery. We focus particular attention on cryo-electron microscopy structures of intact multisubunit tethers in complex with SNAREs or SM proteins, as well as a structure of an SM protein bound to multiple SNAREs. The insights gained from this work emphasize the advantages of studying the fusion machinery intact and in context.

AB - The compartmentalization of eukaryotic cells is reliant on the fidelity of vesicle-mediated intracellular transport. Vesicles deliver their cargo via membrane fusion, a process requiring membrane tethers, Sec1/Munc18 (SM) proteins, and SNAREs. These components function in concert to ensure that membrane fusion is efficient and accurate, but the mechanisms underlying their cooperative action are still in many respects mysterious. In this brief review, we highlight recent progress toward a more integrative understanding of the vesicle fusion machinery. We focus particular attention on cryo-electron microscopy structures of intact multisubunit tethers in complex with SNAREs or SM proteins, as well as a structure of an SM protein bound to multiple SNAREs. The insights gained from this work emphasize the advantages of studying the fusion machinery intact and in context.

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DO - https://doi.org/10.1016/j.ceb.2023.102191

M3 - Review article

C2 - 37421936

SN - 0955-0674

VL - 83

JO - Current Opinion in Cell Biology

JF - Current Opinion in Cell Biology

M1 - 102191

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The machinery of vesicle fusion

Abstract

ASJC Scopus subject areas

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