The molecular weight of bacteriophage θ6 and its nucleocapsid

Loren A. Day, Leonard Mindich

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

A diameter of 82 nm, a molecular weight of 99 × 106, and a refractive index increment of 0.152 g-1cm3 were obtained for θ6 bacteriophage from turbidity measurements made with an analytical ultracentrifuge and a conventional spectrophotometer. Turbidity was also used to obtain a diameter of 64 nm and a molecular weight of 40 × 106 for the θ6 nucleocapsid produced when virus solutions are made 2% Triton X-100. The RNA content found for the intact virus by the orcinol method was 10%- by weight, which corresponds to 10 million for the total molecular weight of the three double-stranded RNA molecules comprising the genome. The phospholipid content of the intact virus was found to be 20% by weight. The relative amounts of individual protein species were determined by gel electrophoretic analyses of radioactive virus. The results yielded estimates of the number of copies of each protein in the virion.

Original languageEnglish (US)
Pages (from-to)376-385
Number of pages10
JournalVirology
Volume103
Issue number2
DOIs
StatePublished - Jan 1 1980

Fingerprint

Nucleocapsid
Bacteriophages
Molecular Weight
Viruses
Weights and Measures
Refractometry
Double-Stranded RNA
Octoxynol
Virion
Phospholipids
Proteins
Gels
Genome
RNA

All Science Journal Classification (ASJC) codes

  • Virology

Cite this

Day, Loren A. ; Mindich, Leonard. / The molecular weight of bacteriophage θ6 and its nucleocapsid. In: Virology. 1980 ; Vol. 103, No. 2. pp. 376-385.
@article{9a1467a4d5c848909e17d6ca202a0c9e,
title = "The molecular weight of bacteriophage θ6 and its nucleocapsid",
abstract = "A diameter of 82 nm, a molecular weight of 99 × 106, and a refractive index increment of 0.152 g-1cm3 were obtained for θ6 bacteriophage from turbidity measurements made with an analytical ultracentrifuge and a conventional spectrophotometer. Turbidity was also used to obtain a diameter of 64 nm and a molecular weight of 40 × 106 for the θ6 nucleocapsid produced when virus solutions are made 2{\%} Triton X-100. The RNA content found for the intact virus by the orcinol method was 10{\%}- by weight, which corresponds to 10 million for the total molecular weight of the three double-stranded RNA molecules comprising the genome. The phospholipid content of the intact virus was found to be 20{\%} by weight. The relative amounts of individual protein species were determined by gel electrophoretic analyses of radioactive virus. The results yielded estimates of the number of copies of each protein in the virion.",
author = "Day, {Loren A.} and Leonard Mindich",
year = "1980",
month = "1",
day = "1",
doi = "https://doi.org/10.1016/0042-6822(80)90196-8",
language = "English (US)",
volume = "103",
pages = "376--385",
journal = "Virology",
issn = "0042-6822",
publisher = "Academic Press Inc.",
number = "2",

}

The molecular weight of bacteriophage θ6 and its nucleocapsid. / Day, Loren A.; Mindich, Leonard.

In: Virology, Vol. 103, No. 2, 01.01.1980, p. 376-385.

Research output: Contribution to journalArticle

TY - JOUR

T1 - The molecular weight of bacteriophage θ6 and its nucleocapsid

AU - Day, Loren A.

AU - Mindich, Leonard

PY - 1980/1/1

Y1 - 1980/1/1

N2 - A diameter of 82 nm, a molecular weight of 99 × 106, and a refractive index increment of 0.152 g-1cm3 were obtained for θ6 bacteriophage from turbidity measurements made with an analytical ultracentrifuge and a conventional spectrophotometer. Turbidity was also used to obtain a diameter of 64 nm and a molecular weight of 40 × 106 for the θ6 nucleocapsid produced when virus solutions are made 2% Triton X-100. The RNA content found for the intact virus by the orcinol method was 10%- by weight, which corresponds to 10 million for the total molecular weight of the three double-stranded RNA molecules comprising the genome. The phospholipid content of the intact virus was found to be 20% by weight. The relative amounts of individual protein species were determined by gel electrophoretic analyses of radioactive virus. The results yielded estimates of the number of copies of each protein in the virion.

AB - A diameter of 82 nm, a molecular weight of 99 × 106, and a refractive index increment of 0.152 g-1cm3 were obtained for θ6 bacteriophage from turbidity measurements made with an analytical ultracentrifuge and a conventional spectrophotometer. Turbidity was also used to obtain a diameter of 64 nm and a molecular weight of 40 × 106 for the θ6 nucleocapsid produced when virus solutions are made 2% Triton X-100. The RNA content found for the intact virus by the orcinol method was 10%- by weight, which corresponds to 10 million for the total molecular weight of the three double-stranded RNA molecules comprising the genome. The phospholipid content of the intact virus was found to be 20% by weight. The relative amounts of individual protein species were determined by gel electrophoretic analyses of radioactive virus. The results yielded estimates of the number of copies of each protein in the virion.

UR - http://www.scopus.com/inward/record.url?scp=0018878878&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0018878878&partnerID=8YFLogxK

U2 - https://doi.org/10.1016/0042-6822(80)90196-8

DO - https://doi.org/10.1016/0042-6822(80)90196-8

M3 - Article

VL - 103

SP - 376

EP - 385

JO - Virology

JF - Virology

SN - 0042-6822

IS - 2

ER -