The role of centromere alignment in meiosis I segregation of homologous chromosomes in Saccharomyces cerevisiae

Cesar E. Guerra, David B. Kaback

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6 Scopus citations

Abstract

Dining meiosis, homologous chromosomes pair and then segregate from each other at the first meiotic division. Homologous centromeres appear to be aligned when chromosomes are paired. The role of centromere alignment in meiotic chromosome segregation was investigated in Saccharomyces cerevisiae diploids that contained one intact copy of chromosome I and one copy bisected into two functional centromere-containing fragments. The centromere on one fragment was aligned with the centromere on the intact chromosome while the centromere on the other fragment was either aligned or misaligned. Fragments containing aligned centromeres segregated efficiently from the intact chromosome, while fragments containing misaligned centromeres segregated much less efficiently from the intact chromosome. Less efficient segregation was correlated with crossing over in the region between the misaligned centromeres. Models that suggest that these crossovers impede proper segregation by preventing either a segregation-promoting chromosome alignment on the meiotic spindle or some physical interaction between homologous centromeres are proposed.

Original languageAmerican English
Pages (from-to)1547-1560
Number of pages14
JournalGenetics
Volume153
Issue number4
StatePublished - Dec 1999

ASJC Scopus subject areas

  • Genetics

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