The role of the loop 1 region in MazFbs mRNA interferase from Bacillus subtilis in recognition of the 3′ end of the RNA substrate

Yojiro Ishida, Keiko Inouye, Masayori Inouye

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

MazFbs is an mRNA interferase from Bacillus subtilis specifically recognizing UACAU. The X-ray structure of its complex with an RNA substrate has been also solved. When its amino acid sequence is compared with that of MazFhw, an mRNA interferase from a highly halophilic archaeon, recognizing UUACUCA, the 9-residue loop-1 region is highly homologous except that the V16V17 sequence in MazFbs is replaced with TK in MazFhw. Thus, we examined the role of the VV sequence in RNA substrate recognition by replacing it with TK, GG, AA or LL. The substitution mutants thus constructed showed significant differences in cleavage specificity using MS2 phage RNA. The primer extension analysis of the cleavage sites revealed that the VV sequence plays an important role in the recognition of the 3′-end base of the RNA substrate.

Original languageEnglish (US)
Pages (from-to)403-408
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume483
Issue number1
DOIs
StatePublished - Jan 29 2017

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Keywords

  • Bacillus subtilis
  • MS2 phage RNA
  • MazF
  • Site-directed mutagenesis

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