The Saccharomyces Pif1p DNA helicase and the highly related Rrm3p have opposite effects on replication fork progression in ribosomal DNA

Andreas Ivessa, Jin Qiu Zhou, Virginia A. Zakian

Research output: Contribution to journalArticle

197 Citations (Scopus)

Abstract

Replication of Saccharomyces ribosomal DNA (rDNA) proceeds bidirectionally from origins in a subset of the ~150 tandem repeats, but the leftward-moving fork stops when it encounters the replication fork barrier (RFB). The Pif1p helicase and the highly related Rrm3p were rDNA associated in vivo. Both proteins affected rDNA replication but had opposing effects on fork progression. Pif1p helped maintain the RFB. Rrm3p appears to be the replicative helicase for rDNA as it acted catalytically to promote fork progression throughout the rDNA. Loss of Rrm3p increased rDNA breakage and accumulation of rDNA circles, whereas breakage and circles were less common in pif1 cells. These data support a model in which replication fork pausing causes breakage and recombination in the rDNA.

Original languageEnglish (US)
Pages (from-to)479-489
Number of pages11
JournalCell
Volume100
Issue number4
DOIs
StatePublished - Feb 18 2000

Fingerprint

DNA Helicases
Saccharomyces
Ribosomal DNA
Tandem Repeat Sequences
DNA Replication
Genetic Recombination
Proteins

Cite this

@article{0180363ad3e34f4aa7bf82534173b5b0,
title = "The Saccharomyces Pif1p DNA helicase and the highly related Rrm3p have opposite effects on replication fork progression in ribosomal DNA",
abstract = "Replication of Saccharomyces ribosomal DNA (rDNA) proceeds bidirectionally from origins in a subset of the ~150 tandem repeats, but the leftward-moving fork stops when it encounters the replication fork barrier (RFB). The Pif1p helicase and the highly related Rrm3p were rDNA associated in vivo. Both proteins affected rDNA replication but had opposing effects on fork progression. Pif1p helped maintain the RFB. Rrm3p appears to be the replicative helicase for rDNA as it acted catalytically to promote fork progression throughout the rDNA. Loss of Rrm3p increased rDNA breakage and accumulation of rDNA circles, whereas breakage and circles were less common in pif1 cells. These data support a model in which replication fork pausing causes breakage and recombination in the rDNA.",
author = "Andreas Ivessa and Zhou, {Jin Qiu} and Zakian, {Virginia A.}",
year = "2000",
month = "2",
day = "18",
doi = "https://doi.org/10.1016/S0092-8674(00)80683-2",
language = "English (US)",
volume = "100",
pages = "479--489",
journal = "Cell",
issn = "0092-8674",
publisher = "Cell Press",
number = "4",

}

The Saccharomyces Pif1p DNA helicase and the highly related Rrm3p have opposite effects on replication fork progression in ribosomal DNA. / Ivessa, Andreas; Zhou, Jin Qiu; Zakian, Virginia A.

In: Cell, Vol. 100, No. 4, 18.02.2000, p. 479-489.

Research output: Contribution to journalArticle

TY - JOUR

T1 - The Saccharomyces Pif1p DNA helicase and the highly related Rrm3p have opposite effects on replication fork progression in ribosomal DNA

AU - Ivessa, Andreas

AU - Zhou, Jin Qiu

AU - Zakian, Virginia A.

PY - 2000/2/18

Y1 - 2000/2/18

N2 - Replication of Saccharomyces ribosomal DNA (rDNA) proceeds bidirectionally from origins in a subset of the ~150 tandem repeats, but the leftward-moving fork stops when it encounters the replication fork barrier (RFB). The Pif1p helicase and the highly related Rrm3p were rDNA associated in vivo. Both proteins affected rDNA replication but had opposing effects on fork progression. Pif1p helped maintain the RFB. Rrm3p appears to be the replicative helicase for rDNA as it acted catalytically to promote fork progression throughout the rDNA. Loss of Rrm3p increased rDNA breakage and accumulation of rDNA circles, whereas breakage and circles were less common in pif1 cells. These data support a model in which replication fork pausing causes breakage and recombination in the rDNA.

AB - Replication of Saccharomyces ribosomal DNA (rDNA) proceeds bidirectionally from origins in a subset of the ~150 tandem repeats, but the leftward-moving fork stops when it encounters the replication fork barrier (RFB). The Pif1p helicase and the highly related Rrm3p were rDNA associated in vivo. Both proteins affected rDNA replication but had opposing effects on fork progression. Pif1p helped maintain the RFB. Rrm3p appears to be the replicative helicase for rDNA as it acted catalytically to promote fork progression throughout the rDNA. Loss of Rrm3p increased rDNA breakage and accumulation of rDNA circles, whereas breakage and circles were less common in pif1 cells. These data support a model in which replication fork pausing causes breakage and recombination in the rDNA.

UR - http://www.scopus.com/inward/record.url?scp=0034681257&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034681257&partnerID=8YFLogxK

U2 - https://doi.org/10.1016/S0092-8674(00)80683-2

DO - https://doi.org/10.1016/S0092-8674(00)80683-2

M3 - Article

C2 - 10693764

VL - 100

SP - 479

EP - 489

JO - Cell

JF - Cell

SN - 0092-8674

IS - 4

ER -