The Use of Tissue Engineering to Fabricate Perfusable 3D Brain Microvessels in vitro

Kalpani N.Udeni Galpayage Dona; Jonathan Franklin Hale; Tobi Salako; Akanksha Anandanatarajan; Kiet A. Tran; Brandon J. DeOre; Peter Adam Galie; Servio Heybert Ramirez; Allison Michelle Andrews

doi:10.3389/fphys.2021.715431

The Use of Tissue Engineering to Fabricate Perfusable 3D Brain Microvessels in vitro

Kalpani N.Udeni Galpayage Dona, Jonathan Franklin Hale, Tobi Salako, Akanksha Anandanatarajan, Kiet A. Tran, Brandon J. DeOre, Peter Adam Galie, Servio Heybert Ramirez, Allison Michelle Andrews

Biomedical Engineering

Rowan University

Research output: Contribution to journal › Article › peer-review

Abstract

Tissue engineering of the blood-brain barrier (BBB) in vitro has been rapidly expanding to address the challenges of mimicking the native structure and function of the BBB. Most of these models utilize 2D conventional microfluidic techniques. However, 3D microvascular models offer the potential to more closely recapitulate the cytoarchitecture and multicellular arrangement of in vivo microvasculature, and also can recreate branching and network topologies of the vascular bed. In this perspective, we discuss current 3D brain microvessel modeling techniques including templating, printing, and self-assembling capillary networks. Furthermore, we address the use of biological matrices and fluid dynamics. Finally, key challenges are identified along with future directions that will improve development of next generation of brain microvasculature models.

Original language	American English
Article number	715431
Journal	Frontiers in Physiology
Volume	12
DOIs	https://doi.org/10.3389/fphys.2021.715431
State	Published - Aug 31 2021

ASJC Scopus subject areas

Physiology
Physiology (medical)

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title = "The Use of Tissue Engineering to Fabricate Perfusable 3D Brain Microvessels in vitro",

abstract = "Tissue engineering of the blood-brain barrier (BBB) in vitro has been rapidly expanding to address the challenges of mimicking the native structure and function of the BBB. Most of these models utilize 2D conventional microfluidic techniques. However, 3D microvascular models offer the potential to more closely recapitulate the cytoarchitecture and multicellular arrangement of in vivo microvasculature, and also can recreate branching and network topologies of the vascular bed. In this perspective, we discuss current 3D brain microvessel modeling techniques including templating, printing, and self-assembling capillary networks. Furthermore, we address the use of biological matrices and fluid dynamics. Finally, key challenges are identified along with future directions that will improve development of next generation of brain microvasculature models.",

author = "{Galpayage Dona}, {Kalpani N.Udeni} and Hale, {Jonathan Franklin} and Tobi Salako and Akanksha Anandanatarajan and Tran, {Kiet A.} and DeOre, {Brandon J.} and Galie, {Peter Adam} and Ramirez, {Servio Heybert} and Andrews, {Allison Michelle}",

note = "Publisher Copyright: {\textcopyright} Copyright {\textcopyright} 2021 Galpayage Dona, Hale, Salako, Anandanatarajan, Tran, DeOre, Galie, Ramirez and Andrews.",

year = "2021",

month = aug,

day = "31",

doi = "10.3389/fphys.2021.715431",

language = "American English",

volume = "12",

journal = "Frontiers in Physiology",

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T1 - The Use of Tissue Engineering to Fabricate Perfusable 3D Brain Microvessels in vitro

AU - Galpayage Dona, Kalpani N.Udeni

AU - Hale, Jonathan Franklin

AU - Salako, Tobi

AU - Anandanatarajan, Akanksha

AU - Tran, Kiet A.

AU - DeOre, Brandon J.

AU - Galie, Peter Adam

AU - Ramirez, Servio Heybert

AU - Andrews, Allison Michelle

PY - 2021/8/31

Y1 - 2021/8/31

N2 - Tissue engineering of the blood-brain barrier (BBB) in vitro has been rapidly expanding to address the challenges of mimicking the native structure and function of the BBB. Most of these models utilize 2D conventional microfluidic techniques. However, 3D microvascular models offer the potential to more closely recapitulate the cytoarchitecture and multicellular arrangement of in vivo microvasculature, and also can recreate branching and network topologies of the vascular bed. In this perspective, we discuss current 3D brain microvessel modeling techniques including templating, printing, and self-assembling capillary networks. Furthermore, we address the use of biological matrices and fluid dynamics. Finally, key challenges are identified along with future directions that will improve development of next generation of brain microvasculature models.

AB - Tissue engineering of the blood-brain barrier (BBB) in vitro has been rapidly expanding to address the challenges of mimicking the native structure and function of the BBB. Most of these models utilize 2D conventional microfluidic techniques. However, 3D microvascular models offer the potential to more closely recapitulate the cytoarchitecture and multicellular arrangement of in vivo microvasculature, and also can recreate branching and network topologies of the vascular bed. In this perspective, we discuss current 3D brain microvessel modeling techniques including templating, printing, and self-assembling capillary networks. Furthermore, we address the use of biological matrices and fluid dynamics. Finally, key challenges are identified along with future directions that will improve development of next generation of brain microvasculature models.

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The Use of Tissue Engineering to Fabricate Perfusable 3D Brain Microvessels in vitro

Abstract

ASJC Scopus subject areas

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