Three stage sample preparation for purification of proteins from complex biological samples

M. Javanmard; S. Emaminejad; R. W. Davis; C. Gupta; R. T. Howe

doi:10.1109/ICSENS.2013.6688315

Three stage sample preparation for purification of proteins from complex biological samples

M. Javanmard
, S. Emaminejad
, R. W. Davis
, C. Gupta
, R. T. Howe

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

3 Scopus citations

Abstract

In this manuscript we present a three-component sample preparation platform for delivering proteins, with controlled orientation, purified from a complex biological sample to a sensor surface. The presented platform depletes highly abundant proteins and cells from complex biological samples, and then is capable of immobilizing the target analyte on the surface with controlled immobilization. A herringbone structure is used to mix the test sample with beads coated with antibodies against the highly abundant proteins, which is then fed into a second component, consisting of a filter trench with interdigitated electrodes above for applying dielectrophoretic force. The beads and cells are pushed down into the filter trench, thus allowing only the serum to pass through. In a third step, protein orientation and immobilization is controlled with electric field. In this manuscript we discuss the proof of concept for each of these three components separately.

Original language	American English
Title of host publication	IEEE SENSORS 2013 - Proceedings
Publisher	IEEE Computer Society
ISBN (Print)	9781467346405
DOIs	https://doi.org/10.1109/ICSENS.2013.6688315
State	Published - 2013
Externally published	Yes
Event	12th IEEE SENSORS 2013 Conference - Baltimore, MD, United States Duration: Nov 4 2013 → Nov 6 2013

Publication series

Name	Proceedings of IEEE Sensors

Other

Other	12th IEEE SENSORS 2013 Conference
Country/Territory	United States
City	Baltimore, MD
Period	11/4/13 → 11/6/13

ASJC Scopus subject areas

Electrical and Electronic Engineering

Access to Document

10.1109/ICSENS.2013.6688315

Cite this

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title = "Three stage sample preparation for purification of proteins from complex biological samples",

abstract = "In this manuscript we present a three-component sample preparation platform for delivering proteins, with controlled orientation, purified from a complex biological sample to a sensor surface. The presented platform depletes highly abundant proteins and cells from complex biological samples, and then is capable of immobilizing the target analyte on the surface with controlled immobilization. A herringbone structure is used to mix the test sample with beads coated with antibodies against the highly abundant proteins, which is then fed into a second component, consisting of a filter trench with interdigitated electrodes above for applying dielectrophoretic force. The beads and cells are pushed down into the filter trench, thus allowing only the serum to pass through. In a third step, protein orientation and immobilization is controlled with electric field. In this manuscript we discuss the proof of concept for each of these three components separately.",

author = "M. Javanmard and S. Emaminejad and Davis, \{R. W.\} and C. Gupta and Howe, \{R. T.\}",

year = "2013",

doi = "10.1109/ICSENS.2013.6688315",

language = "American English",

isbn = "9781467346405",

series = "Proceedings of IEEE Sensors",

publisher = "IEEE Computer Society",

booktitle = "IEEE SENSORS 2013 - Proceedings",

address = "United States",

note = "12th IEEE SENSORS 2013 Conference ; Conference date: 04-11-2013 Through 06-11-2013",

}

Javanmard, M, Emaminejad, S, Davis, RW, Gupta, C & Howe, RT 2013, Three stage sample preparation for purification of proteins from complex biological samples. in IEEE SENSORS 2013 - Proceedings., 6688315, Proceedings of IEEE Sensors, IEEE Computer Society, 12th IEEE SENSORS 2013 Conference, Baltimore, MD, United States, 11/4/13. https://doi.org/10.1109/ICSENS.2013.6688315

TY - GEN

T1 - Three stage sample preparation for purification of proteins from complex biological samples

AU - Javanmard, M.

AU - Emaminejad, S.

AU - Davis, R. W.

AU - Gupta, C.

AU - Howe, R. T.

PY - 2013

Y1 - 2013

N2 - In this manuscript we present a three-component sample preparation platform for delivering proteins, with controlled orientation, purified from a complex biological sample to a sensor surface. The presented platform depletes highly abundant proteins and cells from complex biological samples, and then is capable of immobilizing the target analyte on the surface with controlled immobilization. A herringbone structure is used to mix the test sample with beads coated with antibodies against the highly abundant proteins, which is then fed into a second component, consisting of a filter trench with interdigitated electrodes above for applying dielectrophoretic force. The beads and cells are pushed down into the filter trench, thus allowing only the serum to pass through. In a third step, protein orientation and immobilization is controlled with electric field. In this manuscript we discuss the proof of concept for each of these three components separately.

AB - In this manuscript we present a three-component sample preparation platform for delivering proteins, with controlled orientation, purified from a complex biological sample to a sensor surface. The presented platform depletes highly abundant proteins and cells from complex biological samples, and then is capable of immobilizing the target analyte on the surface with controlled immobilization. A herringbone structure is used to mix the test sample with beads coated with antibodies against the highly abundant proteins, which is then fed into a second component, consisting of a filter trench with interdigitated electrodes above for applying dielectrophoretic force. The beads and cells are pushed down into the filter trench, thus allowing only the serum to pass through. In a third step, protein orientation and immobilization is controlled with electric field. In this manuscript we discuss the proof of concept for each of these three components separately.

UR - https://www.scopus.com/pages/publications/84893975704

UR - https://www.scopus.com/pages/publications/84893975704#tab=citedBy

U2 - 10.1109/ICSENS.2013.6688315

DO - 10.1109/ICSENS.2013.6688315

M3 - Conference contribution

SN - 9781467346405

T3 - Proceedings of IEEE Sensors

BT - IEEE SENSORS 2013 - Proceedings

PB - IEEE Computer Society

T2 - 12th IEEE SENSORS 2013 Conference

Y2 - 4 November 2013 through 6 November 2013

ER -

Three stage sample preparation for purification of proteins from complex biological samples

Abstract

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Other

ASJC Scopus subject areas

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