Transcriptional analysis in the brain: Trophin-induced hippocampal synaptic plasticity

Janet Alder; Smita Thakker-Varia; Ira B. Black

doi:https://doi.org/10.1023/A:1020909022145

Transcriptional analysis in the brain: Trophin-induced hippocampal synaptic plasticity

Janet Alder, Smita Thakker-Varia, Ira B. Black

Rutgers, The State University

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Gene profiling in the central nervous system presents unique challenges due to the unprecedented heterogeneity of cells, systems and functions in time and space. We have employed a multidisciplinary approach using whole cell patch clamp recording and transcriptional analysis to define the genomic basis of trophin-induced hippocampal synaptic plasticity. Transcriptional analysis of single cells by linear amplification of antisense RNA has added a new dimension of sensitivity and selectivity to the study of the complex and heterogeneous population of neurons. We describe different gene expression profiling techniques that offer novel approaches to monitoring thousands of genes in parallel, fostering identification of circuits involved in learning and memory.

Original language	English (US)
Pages (from-to)	1079-1092
Number of pages	14
Journal	Neurochemical Research
Volume	27
Issue number	10
DOIs	https://doi.org/10.1023/A:1020909022145
State	Published - Oct 1 2002

ASJC Scopus subject areas

Biochemistry
Cellular and Molecular Neuroscience

Keywords

Brain derived neurotrophic factor “BDNF”
Differential display
Gene profiling
Synaptic plasticity, microarray

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https://doi.org/10.1023/A:1020909022145

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title = "Transcriptional analysis in the brain: Trophin-induced hippocampal synaptic plasticity",

abstract = "Gene profiling in the central nervous system presents unique challenges due to the unprecedented heterogeneity of cells, systems and functions in time and space. We have employed a multidisciplinary approach using whole cell patch clamp recording and transcriptional analysis to define the genomic basis of trophin-induced hippocampal synaptic plasticity. Transcriptional analysis of single cells by linear amplification of antisense RNA has added a new dimension of sensitivity and selectivity to the study of the complex and heterogeneous population of neurons. We describe different gene expression profiling techniques that offer novel approaches to monitoring thousands of genes in parallel, fostering identification of circuits involved in learning and memory.",

keywords = "Brain derived neurotrophic factor “BDNF”, Differential display, Gene profiling, Synaptic plasticity, microarray",

author = "Janet Alder and Smita Thakker-Varia and Black, {Ira B.}",

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T2 - Trophin-induced hippocampal synaptic plasticity

AU - Alder, Janet

AU - Thakker-Varia, Smita

AU - Black, Ira B.

N1 - Funding Information: This work was supported by grants from NARSAD, the UMDNJ Foundation, NICHD, and the NJ Commission for Science & Technology.

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AB - Gene profiling in the central nervous system presents unique challenges due to the unprecedented heterogeneity of cells, systems and functions in time and space. We have employed a multidisciplinary approach using whole cell patch clamp recording and transcriptional analysis to define the genomic basis of trophin-induced hippocampal synaptic plasticity. Transcriptional analysis of single cells by linear amplification of antisense RNA has added a new dimension of sensitivity and selectivity to the study of the complex and heterogeneous population of neurons. We describe different gene expression profiling techniques that offer novel approaches to monitoring thousands of genes in parallel, fostering identification of circuits involved in learning and memory.

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KW - Differential display

KW - Gene profiling

KW - Synaptic plasticity, microarray

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Transcriptional analysis in the brain: Trophin-induced hippocampal synaptic plasticity

Abstract

ASJC Scopus subject areas

Keywords

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