Transcriptomic alterations in Trypanosoma cruzi-infected cardiac myocytes

TY - JOUR

T1 - Transcriptomic alterations in Trypanosoma cruzi-infected cardiac myocytes

AU - Goldenberg, Regina Coeli dos Santos

AU - Iacobas, Dumitru A.

AU - Iacobas, Sanda

AU - Rocha, Leonardo Lima

AU - da Silva de Azevedo Fortes, Fabio

AU - Vairo, Leandro

AU - Nagajyothi, Fnu

AU - Campos de Carvalho, Antonio Carlos

AU - Tanowitz, Herbert B.

AU - Spray, David C.

N1 - Funding Information: Support was from National Institutes of Health (HL-73732, HD-32573, AI-076248, AI-052739); CAPES; CNPq and FAPERJ. RCSG, LLS and FSAF were supported by a Fogarty International Training Grant D43TW007129

PY - 2009/12

Y1 - 2009/12

N2 - Trypanosoma cruzi infection is a major cause of cardiomyopathy. Previous gene profiling studies of infected mouse hearts have revealed prominent changes in gene expression within many functional pathways. This variety of transcriptomic changes in infected mice raises the question of whether gene expression alterations in whole hearts are due to changes in infected cardiac myocytes or other cells or even to systemic effects of the infection on the heart. We employed microarrays to examine infected cardiac myocyte cultures 48 h post-infection. Statistical comparison of gene expression levels of 7624 well annotated unigenes in four independent cultures of infected and uninfected myocytes detected substantial (≥1.5 absolute fold changes) in 420 (5.5%) of the sampled genes. Major categories of affected genes included those involved in immune response, extracellular matrix and cell adhesion. These findings on infected cardiac myocytes in culture reveal that alterations in cardiac gene expression described in Chagas disease are the consequence of both direct infection of the myocytes themselves as well as resulting from the presence of other cell types in the myocardium and systemic effects of infection.

AB - Trypanosoma cruzi infection is a major cause of cardiomyopathy. Previous gene profiling studies of infected mouse hearts have revealed prominent changes in gene expression within many functional pathways. This variety of transcriptomic changes in infected mice raises the question of whether gene expression alterations in whole hearts are due to changes in infected cardiac myocytes or other cells or even to systemic effects of the infection on the heart. We employed microarrays to examine infected cardiac myocyte cultures 48 h post-infection. Statistical comparison of gene expression levels of 7624 well annotated unigenes in four independent cultures of infected and uninfected myocytes detected substantial (≥1.5 absolute fold changes) in 420 (5.5%) of the sampled genes. Major categories of affected genes included those involved in immune response, extracellular matrix and cell adhesion. These findings on infected cardiac myocytes in culture reveal that alterations in cardiac gene expression described in Chagas disease are the consequence of both direct infection of the myocytes themselves as well as resulting from the presence of other cell types in the myocardium and systemic effects of infection.

KW - Cardiac myocyte culture

KW - Cardiomyopathy

KW - Chagas disease

KW - Microarray

KW - Trypanosoma cruzi

UR - https://www.scopus.com/pages/publications/71649104746

UR - https://www.scopus.com/pages/publications/71649104746#tab=citedBy

U2 - 10.1016/j.micinf.2009.08.009

DO - 10.1016/j.micinf.2009.08.009

M3 - Article

C2 - 19729072

SN - 1286-4579

VL - 11

SP - 1140

EP - 1149

JO - Microbes and Infection

JF - Microbes and Infection

IS - 14-15

ER -