Translocation of σ70 with RNA polymerase during transcription: Fluorescence resonance energy transfer assay for movement relative to DNA

Jayanta Mukhopadhyay, Achillefs N. Kapanidis, Vladimir Mekler, Ekaterine Kortkhonjia, Yon W. Ebright, Richard H. Ebright

Research output: Contribution to journalArticlepeer-review

149 Scopus citations

Abstract

Using fluorescence resonance energy transfer, we show that, in the majority of transcription complexes, σ70 is not released from RNA polymerase upon transition from initiation to elongation, but, instead, remains associated with RNA polymerase and translocates with RNA polymerase. The results argue against the presumption that there are necessary subunit-composition differences, and corresponding necessary mechanistic differences, in initiation and elongation. The methods of this report should be generalizable to monitor movement of any molecule relative to any nucleic acid.

Original languageEnglish (US)
Pages (from-to)453-463
Number of pages11
JournalCell
Volume106
Issue number4
DOIs
StatePublished - Aug 24 2001

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

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