Using fluorescence resonance energy transfer, we show that, in the majority of transcription complexes, σ70 is not released from RNA polymerase upon transition from initiation to elongation, but, instead, remains associated with RNA polymerase and translocates with RNA polymerase. The results argue against the presumption that there are necessary subunit-composition differences, and corresponding necessary mechanistic differences, in initiation and elongation. The methods of this report should be generalizable to monitor movement of any molecule relative to any nucleic acid.
All Science Journal Classification (ASJC) codes
- Biochemistry, Genetics and Molecular Biology(all)