Transport of lipopolysaccharide across the cell envelope: The long road of discovery

Natividad Ruiz; Daniel Kahne; Thomas J. Silhavy

doi:https://doi.org/10.1038/nrmicro2184

Transport of lipopolysaccharide across the cell envelope: The long road of discovery

Natividad Ruiz, Daniel Kahne, Thomas J. Silhavy

Molecular Biology

Princeton University

Research output: Contribution to journal › Review article › peer-review

172 Scopus citations

Abstract

Intracellular lipid transport is poorly understood. Genetic studies to identify lipid-transport factors are complicated by the essentiality of many lipids, whereas biochemical and cell biology approaches aiming to determine localization and mechanisms of lipid transport are often challenged by the lack of adequate technology. Here, we review the epic history of how different approaches, technological advances and ingenuity contributed to the recent discovery of a multi-protein pathway that transports lipopolysaccharide across the envelope of Gram-negative bacteria.

Original language	American English
Pages (from-to)	677-683
Number of pages	7
Journal	Nature Reviews Microbiology
Volume	7
Issue number	9
DOIs	https://doi.org/10.1038/nrmicro2184
State	Published - 2009

ASJC Scopus subject areas

Microbiology
Immunology and Microbiology(all)
Infectious Diseases

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https://doi.org/10.1038/nrmicro2184

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abstract = "Intracellular lipid transport is poorly understood. Genetic studies to identify lipid-transport factors are complicated by the essentiality of many lipids, whereas biochemical and cell biology approaches aiming to determine localization and mechanisms of lipid transport are often challenged by the lack of adequate technology. Here, we review the epic history of how different approaches, technological advances and ingenuity contributed to the recent discovery of a multi-protein pathway that transports lipopolysaccharide across the envelope of Gram-negative bacteria.",

author = "Natividad Ruiz and Daniel Kahne and Silhavy, {Thomas J.}",

note = "Funding Information: This work was supported by National Institute of General Medical Sciences Grants GM34821 (to T.J.S.) and GM66174 (to D.K.).",

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AB - Intracellular lipid transport is poorly understood. Genetic studies to identify lipid-transport factors are complicated by the essentiality of many lipids, whereas biochemical and cell biology approaches aiming to determine localization and mechanisms of lipid transport are often challenged by the lack of adequate technology. Here, we review the epic history of how different approaches, technological advances and ingenuity contributed to the recent discovery of a multi-protein pathway that transports lipopolysaccharide across the envelope of Gram-negative bacteria.

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Transport of lipopolysaccharide across the cell envelope: The long road of discovery

Abstract

ASJC Scopus subject areas

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