Trichloroethylene degradation by Escherichia coli containing the cloned Pseudomonas putida F1 toluene dioxygenase genes

Gerben Zylstra, L. P. Wackett, D. T. Gibson

Research output: Contribution to journalArticle

81 Citations (Scopus)

Abstract

Toluene dioxygenase from Pseudomonas putida F1 has been implicated as an enzyme capable of degrading trichloroethylene. This has now been confirmed with Escherichia coli JM109(pDTG601) that contains the structural genes (todC1C2BA) of toluene dioxygenase under the control of the tac promoter. The extent of trichloroethylene degradation by the recombinant organism depended on the cell concentration and the concentration of trichloroethylene. A linear rate of trichloroethylene degradation was observed with the E. coli recombinant strain. In contrast, P. putida F39/D, a mutant strain of P. putida F1 that does not contain cis-toluene dihydrodiol dehydrogenase, showed a much faster initial rate of trichloroethylene degradation which decreased over time.

Original languageEnglish (US)
Pages (from-to)3162-3166
Number of pages5
JournalApplied and environmental microbiology
Volume55
Issue number12
StatePublished - Dec 21 1989
Externally publishedYes

Fingerprint

trichloroethylene
Trichloroethylene
Pseudomonas putida
toluene
Escherichia coli
degradation
gene
Genes
genes
structural genes
promoter regions
toluene dioxygenase
enzyme
mutants
organisms
Enzymes
enzymes
cells

All Science Journal Classification (ASJC) codes

  • Environmental Science(all)
  • Biotechnology
  • Microbiology

Cite this

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Trichloroethylene degradation by Escherichia coli containing the cloned Pseudomonas putida F1 toluene dioxygenase genes. / Zylstra, Gerben; Wackett, L. P.; Gibson, D. T.

In: Applied and environmental microbiology, Vol. 55, No. 12, 21.12.1989, p. 3162-3166.

Research output: Contribution to journalArticle

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