TRPM7, a novel regulator of actomyosin contractility and cell adhesion

Kristopher Clark, Michiel Langeslag, Bart Van Leeuwen, Leonie Ran, Alexey G. Ryazanov, Carl G. Figdor, Wouter H. Moolenaar, Kees Jalink, Frank N. Van Leeuwen

Research output: Contribution to journalArticlepeer-review

269 Scopus citations

Abstract

Actomyosin contractility regulates various cell biological processes including cytokinesis, adhesion and migration. While in lower eukaryotes, α-kinases control actomyosin relaxation, a similar role for mammalian α-kinases has yet to be established. Here, we examined whether TRPM7, a cation channel fused to an α-kinase, can affect actomyosin function. We demonstrate that activation of TRPM7 by bradykinin leads to a Ca2+- and kinase-dependent interaction with the actomyosin cytoskeleton. Moreover, TRPM7 phosphorylates the myosin IIA heavy chain. Accordingly, low overexpression of TRPM7 increases intracellular Ca2+ levels accompanied by cell spreading, adhesion and the formation of focal adhesions. Activation of TRPM7 induces the transformation of these focal adhesions into podosomes by a kinase-dependent mechanism, an effect that can be mimicked by pharmacological inhibition of myosin II. Collectively, our results demonstrate that regulation of cell adhesion by TRPM7 is the combined effect of kinase-dependent and -independent pathways on actomyosin contractility.

Original languageEnglish (US)
Pages (from-to)290-301
Number of pages12
JournalEMBO Journal
Volume25
Issue number2
DOIs
StatePublished - Jan 25 2006

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Molecular Biology
  • Neuroscience(all)

Keywords

  • Cell adhesion
  • Cytoskeleton
  • Myosin
  • Phosphorylation
  • TRPM7

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