Tudor-SN-mediated endonucleolytic decay of human cell microRNAs promotes G1/S phase transition

Reyad A. Elbarbary, Keita Miyoshi, Jason R. Myers, Peicheng Du, John M. Ashton, Bin Tian, Lynne E. Maquat

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

MicroRNAs (miRNAs) are small noncoding RNAs that regulate gene expression. The pathways that mediate mature miRNA decay are less well understood than those that mediate miRNA biogenesis.We found that functional miRNAs are degraded in human cells by the endonuclease Tudor-SN (TSN). In vitro, recombinant TSN initiated the decay of both protein-free and Argonaute 2-loaded miRNAs via endonucleolytic cleavage at CA and UA dinucleotides, preferentially at scissile bonds located more than five nucleotides away from miRNA ends. Cellular targets of TSN-mediated decay defined using microRNA sequencing followed this rule. Inhibiting TSN-mediated miRNA decay by CRISPR-Cas9 knockout of TSN inhibited cell cycle progression by up-regulating a cohort of miRNAs that down-regulates mRNAs that encode proteins critical for the G1-to-S phase transition. Our study indicates that targeting TSN nuclease activity could inhibit pathological cell proliferation.

Original languageEnglish (US)
Pages (from-to)859-862
Number of pages4
JournalScience
Volume356
Issue number6340
DOIs
StatePublished - May 26 2017

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Phase Transition
G1 Phase
MicroRNAs
S Phase
Argonaute Proteins
Clustered Regularly Interspaced Short Palindromic Repeats
Small Untranslated RNA
Endonucleases
Cell Cycle
Down-Regulation
Nucleotides
Cell Proliferation
Gene Expression
Messenger RNA

All Science Journal Classification (ASJC) codes

  • General

Cite this

Elbarbary, R. A., Miyoshi, K., Myers, J. R., Du, P., Ashton, J. M., Tian, B., & Maquat, L. E. (2017). Tudor-SN-mediated endonucleolytic decay of human cell microRNAs promotes G1/S phase transition. Science, 356(6340), 859-862. https://doi.org/10.1126/science.aai9372
Elbarbary, Reyad A. ; Miyoshi, Keita ; Myers, Jason R. ; Du, Peicheng ; Ashton, John M. ; Tian, Bin ; Maquat, Lynne E. / Tudor-SN-mediated endonucleolytic decay of human cell microRNAs promotes G1/S phase transition. In: Science. 2017 ; Vol. 356, No. 6340. pp. 859-862.
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Elbarbary, RA, Miyoshi, K, Myers, JR, Du, P, Ashton, JM, Tian, B & Maquat, LE 2017, 'Tudor-SN-mediated endonucleolytic decay of human cell microRNAs promotes G1/S phase transition', Science, vol. 356, no. 6340, pp. 859-862. https://doi.org/10.1126/science.aai9372

Tudor-SN-mediated endonucleolytic decay of human cell microRNAs promotes G1/S phase transition. / Elbarbary, Reyad A.; Miyoshi, Keita; Myers, Jason R.; Du, Peicheng; Ashton, John M.; Tian, Bin; Maquat, Lynne E.

In: Science, Vol. 356, No. 6340, 26.05.2017, p. 859-862.

Research output: Contribution to journalArticle

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AU - Elbarbary, Reyad A.

AU - Miyoshi, Keita

AU - Myers, Jason R.

AU - Du, Peicheng

AU - Ashton, John M.

AU - Tian, Bin

AU - Maquat, Lynne E.

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