Two-Photon Absorption of Cationic Conjugated Polyelectrolytes: Effects of Aggregation and Application to 2-Photon-Sensitized Fluorescence from Green Fluorescent Protein

Shanshan Wang; Zhiliang Li; Xinglei Liu; Samantha Phan; Fengting Lv; Kevin D. Belfield; Shu Wang; Kirk S. Schanze

doi:10.1021/acs.chemmater.7b00676

Two-Photon Absorption of Cationic Conjugated Polyelectrolytes: Effects of Aggregation and Application to 2-Photon-Sensitized Fluorescence from Green Fluorescent Protein

Shanshan Wang, Zhiliang Li, Xinglei Liu, Samantha Phan, Fengting Lv, Kevin D. Belfield, Shu Wang, Kirk S. Schanze

New Jersey Institute of Technology

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

Two cationic conjugated polyelectrolytes (CPEs) based on poly(phenylene ethynylene) are found to exhibit efficient 2-photon excited fluorescence. In particular, the CPE aggregates display enhanced 2-photon absorption compared to the nonaggregated states. The CPEs are used as 2-photon absorption sensitizers to harvest light and amplify the fluorescence from green fluorescent protein (GFP) through Förster resonance energy transfer. Considerably enhanced GFP fluorescence under near-infrared 2-photon excitation is demonstrated both in solution and in HeLa cells. The results suggest applications in 2-photon fluorescence imaging of cells, tissues, and organs of living animals expressing GFP.

Original language	American English
Pages (from-to)	3295-3303
Number of pages	9
Journal	Chemistry of Materials
Volume	29
Issue number	7
DOIs	https://doi.org/10.1021/acs.chemmater.7b00676
State	Published - Apr 11 2017

ASJC Scopus subject areas

General Chemistry
General Chemical Engineering
Materials Chemistry

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title = "Two-Photon Absorption of Cationic Conjugated Polyelectrolytes: Effects of Aggregation and Application to 2-Photon-Sensitized Fluorescence from Green Fluorescent Protein",

abstract = "Two cationic conjugated polyelectrolytes (CPEs) based on poly(phenylene ethynylene) are found to exhibit efficient 2-photon excited fluorescence. In particular, the CPE aggregates display enhanced 2-photon absorption compared to the nonaggregated states. The CPEs are used as 2-photon absorption sensitizers to harvest light and amplify the fluorescence from green fluorescent protein (GFP) through F{\"o}rster resonance energy transfer. Considerably enhanced GFP fluorescence under near-infrared 2-photon excitation is demonstrated both in solution and in HeLa cells. The results suggest applications in 2-photon fluorescence imaging of cells, tissues, and organs of living animals expressing GFP.",

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T2 - Effects of Aggregation and Application to 2-Photon-Sensitized Fluorescence from Green Fluorescent Protein

AU - Wang, Shanshan

AU - Li, Zhiliang

AU - Liu, Xinglei

AU - Phan, Samantha

AU - Lv, Fengting

AU - Belfield, Kevin D.

AU - Wang, Shu

AU - Schanze, Kirk S.

PY - 2017/4/11

Y1 - 2017/4/11

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AB - Two cationic conjugated polyelectrolytes (CPEs) based on poly(phenylene ethynylene) are found to exhibit efficient 2-photon excited fluorescence. In particular, the CPE aggregates display enhanced 2-photon absorption compared to the nonaggregated states. The CPEs are used as 2-photon absorption sensitizers to harvest light and amplify the fluorescence from green fluorescent protein (GFP) through Förster resonance energy transfer. Considerably enhanced GFP fluorescence under near-infrared 2-photon excitation is demonstrated both in solution and in HeLa cells. The results suggest applications in 2-photon fluorescence imaging of cells, tissues, and organs of living animals expressing GFP.

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Two-Photon Absorption of Cationic Conjugated Polyelectrolytes: Effects of Aggregation and Application to 2-Photon-Sensitized Fluorescence from Green Fluorescent Protein

Abstract

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