Type-2 dominant cytokine gene expression following hepatic surgery

TY - JOUR

T1 - Type-2 dominant cytokine gene expression following hepatic surgery

AU - Khatri, Vijay P.

AU - Shah, Manisha H.

AU - Petrelli, Nicholas J.

AU - Li, Yueju

AU - Beckett, Laurel

AU - Gibbs, John F.

AU - Rodriguez-Bigas, Miguel A.

PY - 2006/9

Y1 - 2006/9

N2 - Background/Purpose: Hemorrhage and ischemic liver injuries associated with hepatic resection are thought to play a role in postoperative complications, possibly through altered cytokine production. The current study was performed to investigate the effects of hepatectomy on cytokine gene expression. Methods: We collected blood preoperatively, at completion of operation, and on postoperative days 1 and 5 from ten patients undergoing hepatic resection. The peripheral blood mononuclear cells were evaluated with real-time quantitative reverse transcription-polymerase chain reaction (RT-PCR) for gene expression of interleukin-10 (IL-10), proinflammatory cytokines (interferon-γ [IFNγ ], IL-15, tumor necrosis factor α [TNFα], and chemokines regulated on activation, normal T expressed and secreted [RANTES ], macrophage inflammatory protein 1 alpha [ MIP-1β], [MIP-1β ]). Wilcoxon Rank and paired t-tests were used for statistical analysis. Results: Immediately following hepatectomy there was a significant (31.4 ± 60.5-fold; P < 0.05) increase in IL-10 gene expression that was sustained until the first postoperative day. In contrast, there was a significant downregulation (38 ± 71 eight fold lower than preoperative; P < 0.05) of IFNγ gene expression on day 1. By postoperative day 5, the changes in gene transcript levels of both IL-10 and IFNγ had returned to the preoperative baselines. This contrasting change in IL-10 and IFNγ gene expression in response to hepatic resection was statistically significant (P = 0.02). Conclusions: Hepatectomy elicits an imbalance towards the immunosuppressive type-2 cytokine profile in the early postoperative period. Measurement of cytokine gene transcripts following hepatic resection may have predictive value for clinical outcome, and deserves further study.

AB - Background/Purpose: Hemorrhage and ischemic liver injuries associated with hepatic resection are thought to play a role in postoperative complications, possibly through altered cytokine production. The current study was performed to investigate the effects of hepatectomy on cytokine gene expression. Methods: We collected blood preoperatively, at completion of operation, and on postoperative days 1 and 5 from ten patients undergoing hepatic resection. The peripheral blood mononuclear cells were evaluated with real-time quantitative reverse transcription-polymerase chain reaction (RT-PCR) for gene expression of interleukin-10 (IL-10), proinflammatory cytokines (interferon-γ [IFNγ ], IL-15, tumor necrosis factor α [TNFα], and chemokines regulated on activation, normal T expressed and secreted [RANTES ], macrophage inflammatory protein 1 alpha [ MIP-1β], [MIP-1β ]). Wilcoxon Rank and paired t-tests were used for statistical analysis. Results: Immediately following hepatectomy there was a significant (31.4 ± 60.5-fold; P < 0.05) increase in IL-10 gene expression that was sustained until the first postoperative day. In contrast, there was a significant downregulation (38 ± 71 eight fold lower than preoperative; P < 0.05) of IFNγ gene expression on day 1. By postoperative day 5, the changes in gene transcript levels of both IL-10 and IFNγ had returned to the preoperative baselines. This contrasting change in IL-10 and IFNγ gene expression in response to hepatic resection was statistically significant (P = 0.02). Conclusions: Hepatectomy elicits an imbalance towards the immunosuppressive type-2 cytokine profile in the early postoperative period. Measurement of cytokine gene transcripts following hepatic resection may have predictive value for clinical outcome, and deserves further study.

KW - Chemokines

KW - Cytokine

KW - Hepatic resection

KW - Interferon-gamma

KW - Interleukin-10

UR - https://www.scopus.com/pages/publications/33749233965

U2 - 10.1007/s00534-006-1103-7

DO - 10.1007/s00534-006-1103-7

M3 - Article

C2 - 17013720

SN - 0944-1166

VL - 13

SP - 442

EP - 449

JO - Journal of Hepato-Biliary-Pancreatic Surgery

JF - Journal of Hepato-Biliary-Pancreatic Surgery

IS - 5

ER -