Abstract
This report describes the use of a recombinant murine retrovirus encoding β-galactosidase (PLJ β-gal retrovirus) to study the antiretroviral activity of zidovudine (AZT) and other nucleoside analogs. The PLJ β-gal virus permits the rapid and unequivocal identification of individual virus-infected cells arising from a single cycle of viral replication. With this model system, AZT is shown to completely and irreversibly prevent retrovirus infection of proliferating cell lines as measured by a lack of reporter gene expression. On the other hand, AZT is less effective in protecting growth-arrested cells from retroviral infection. Recombinant retroviruses such as the PLJ β-gal virus are potentially useful reagents for the identification and characterization of antiretroviral compounds.
| Original language | American English |
|---|---|
| Pages (from-to) | 6339-6342 |
| Number of pages | 4 |
| Journal | Journal of virology |
| Volume | 65 |
| Issue number | 11 |
| State | Published - Nov 1991 |
| Externally published | Yes |
ASJC Scopus subject areas
- Microbiology
- Immunology
- Insect Science
- Virology