Use of revertant cell lines to identify targets of v-fos transformation-specific alterations in gene expression.

C. D. Hoemann, Helmut Zarbl

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Two proteins expressed in Rat-1 cells which are targets for v-fos transformation-specific alterations in gene expression were identified as alpha 1(I) and alpha 2(I) procollagen. While procollagen (I) proteins were synthesized in Rat-1 fibroblasts, their synthesis was dramatically reduced in Rat-1 cells transformed with the FBJ-v-fos oncogene. Revertant cell lines, which were previously shown to express a functional fos oncoprotein, resumed the synthesis of procollagen (I) at levels comparable to those seen in Rat-1 cells. Further results indicated that these procollagen proteins were also synthesized in Rat-1 cell lines that constitutively express high levels of a transfected c-fos protooncogene. Together, these observations suggested that constitutive fos protein expression was not sufficient to inhibit synthesis of these proteins. We have further demonstrated that Rat-1 cells transformed by most other oncogenes express abundant levels of procollagen (I), indicating that inhibition of procollagen (I) synthesis is not a general characteristic of transformed Rat-1 cells but is specifically associated with FBJ-v-fos-induced transformation. Northern blot analysis and runoff transcription assay data indicated that the alpha 1(I) procollagen, but not alpha 2(I) procollagen, is differentially regulated at the transcriptional level in Rat-1 fibroblasts, v-fos transformants, and revertants.

Original languageEnglish (US)
Pages (from-to)581-590
Number of pages10
JournalCell growth & differentiation : the molecular biology journal of the American Association for Cancer Research
Volume1
Issue number12
StatePublished - Jan 1 1990
Externally publishedYes

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Procollagen
Gene Expression
Cell Line
Proteins
Fibroblasts
fos Genes
Oncogene Proteins
Oncogenes
Northern Blotting

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

Cite this

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title = "Use of revertant cell lines to identify targets of v-fos transformation-specific alterations in gene expression.",
abstract = "Two proteins expressed in Rat-1 cells which are targets for v-fos transformation-specific alterations in gene expression were identified as alpha 1(I) and alpha 2(I) procollagen. While procollagen (I) proteins were synthesized in Rat-1 fibroblasts, their synthesis was dramatically reduced in Rat-1 cells transformed with the FBJ-v-fos oncogene. Revertant cell lines, which were previously shown to express a functional fos oncoprotein, resumed the synthesis of procollagen (I) at levels comparable to those seen in Rat-1 cells. Further results indicated that these procollagen proteins were also synthesized in Rat-1 cell lines that constitutively express high levels of a transfected c-fos protooncogene. Together, these observations suggested that constitutive fos protein expression was not sufficient to inhibit synthesis of these proteins. We have further demonstrated that Rat-1 cells transformed by most other oncogenes express abundant levels of procollagen (I), indicating that inhibition of procollagen (I) synthesis is not a general characteristic of transformed Rat-1 cells but is specifically associated with FBJ-v-fos-induced transformation. Northern blot analysis and runoff transcription assay data indicated that the alpha 1(I) procollagen, but not alpha 2(I) procollagen, is differentially regulated at the transcriptional level in Rat-1 fibroblasts, v-fos transformants, and revertants.",
author = "Hoemann, {C. D.} and Helmut Zarbl",
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AU - Hoemann, C. D.

AU - Zarbl, Helmut

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N2 - Two proteins expressed in Rat-1 cells which are targets for v-fos transformation-specific alterations in gene expression were identified as alpha 1(I) and alpha 2(I) procollagen. While procollagen (I) proteins were synthesized in Rat-1 fibroblasts, their synthesis was dramatically reduced in Rat-1 cells transformed with the FBJ-v-fos oncogene. Revertant cell lines, which were previously shown to express a functional fos oncoprotein, resumed the synthesis of procollagen (I) at levels comparable to those seen in Rat-1 cells. Further results indicated that these procollagen proteins were also synthesized in Rat-1 cell lines that constitutively express high levels of a transfected c-fos protooncogene. Together, these observations suggested that constitutive fos protein expression was not sufficient to inhibit synthesis of these proteins. We have further demonstrated that Rat-1 cells transformed by most other oncogenes express abundant levels of procollagen (I), indicating that inhibition of procollagen (I) synthesis is not a general characteristic of transformed Rat-1 cells but is specifically associated with FBJ-v-fos-induced transformation. Northern blot analysis and runoff transcription assay data indicated that the alpha 1(I) procollagen, but not alpha 2(I) procollagen, is differentially regulated at the transcriptional level in Rat-1 fibroblasts, v-fos transformants, and revertants.

AB - Two proteins expressed in Rat-1 cells which are targets for v-fos transformation-specific alterations in gene expression were identified as alpha 1(I) and alpha 2(I) procollagen. While procollagen (I) proteins were synthesized in Rat-1 fibroblasts, their synthesis was dramatically reduced in Rat-1 cells transformed with the FBJ-v-fos oncogene. Revertant cell lines, which were previously shown to express a functional fos oncoprotein, resumed the synthesis of procollagen (I) at levels comparable to those seen in Rat-1 cells. Further results indicated that these procollagen proteins were also synthesized in Rat-1 cell lines that constitutively express high levels of a transfected c-fos protooncogene. Together, these observations suggested that constitutive fos protein expression was not sufficient to inhibit synthesis of these proteins. We have further demonstrated that Rat-1 cells transformed by most other oncogenes express abundant levels of procollagen (I), indicating that inhibition of procollagen (I) synthesis is not a general characteristic of transformed Rat-1 cells but is specifically associated with FBJ-v-fos-induced transformation. Northern blot analysis and runoff transcription assay data indicated that the alpha 1(I) procollagen, but not alpha 2(I) procollagen, is differentially regulated at the transcriptional level in Rat-1 fibroblasts, v-fos transformants, and revertants.

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