TY - JOUR
T1 - Use of salicylic acid polymers and bone morphogenetic protein-2 to promote bone regeneration in rabbit parietal bone defects
AU - Mitchell, Ashley
AU - Kim, Brian
AU - Snyder, Sabrina
AU - Subramanian, Sangeeta
AU - Uhrich, Kathryn
AU - O'Connor, J. Patrick
N1 - Funding Information: The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This research was supported by Award Number R01DE019926 from the National Institute of Dental & Craniofacial Research. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institute of Dental & Craniofacial Research or the National Institutes of Health. Publisher Copyright: © SAGE Publications.
PY - 2016/3/1
Y1 - 2016/3/1
N2 - The ability of bone defects to heal spontaneously is inversely related to the size of the defect, such that defects larger than a critical size will not heal without additional therapeutic intervention. Typically, large bone defects are filled with autologous bone harvested from another skeletal site, an osteoconductive bone graft material, treated with an osteoinductive factor such as bone morphogenetic protein-2, or by a combination of these approaches. Despite these interventions, unsatisfactory success and complication rates show that alternative treatment methods are needed. Here, we test whether salicylic acid polymers can be used as guided bone regeneration barriers in conjunction with bone morphogenetic protein-2 to treat 1-cm-diameter defects in rabbit parietal bones. Porous, 1-cm round polycaprolactone scaffolds were infused with calcium sulfate-containing bone morphogenetic protein-2 and then capped on one side with salicylic acid polymers. The polymers slowed resorption of calcium sulfate that was used as a carrier for bone morphogenetic protein-2, indicating that bone morphogenetic protein-2 release into the parietal bone defect was extended by the use of the salicylic acid polymer. Microcomputerized tomography and histomorphometric analysis of the parietal bones 8 weeks after implantation showed that the salicylic acid polymer did not impair bone formation in the defect. These observations indicate that salicylic polymers paired with bone morphogenetic protein-2 can be optimized for use in guided bone regeneration to help repair large bone defects.
AB - The ability of bone defects to heal spontaneously is inversely related to the size of the defect, such that defects larger than a critical size will not heal without additional therapeutic intervention. Typically, large bone defects are filled with autologous bone harvested from another skeletal site, an osteoconductive bone graft material, treated with an osteoinductive factor such as bone morphogenetic protein-2, or by a combination of these approaches. Despite these interventions, unsatisfactory success and complication rates show that alternative treatment methods are needed. Here, we test whether salicylic acid polymers can be used as guided bone regeneration barriers in conjunction with bone morphogenetic protein-2 to treat 1-cm-diameter defects in rabbit parietal bones. Porous, 1-cm round polycaprolactone scaffolds were infused with calcium sulfate-containing bone morphogenetic protein-2 and then capped on one side with salicylic acid polymers. The polymers slowed resorption of calcium sulfate that was used as a carrier for bone morphogenetic protein-2, indicating that bone morphogenetic protein-2 release into the parietal bone defect was extended by the use of the salicylic acid polymer. Microcomputerized tomography and histomorphometric analysis of the parietal bones 8 weeks after implantation showed that the salicylic acid polymer did not impair bone formation in the defect. These observations indicate that salicylic polymers paired with bone morphogenetic protein-2 can be optimized for use in guided bone regeneration to help repair large bone defects.
KW - Tissue regeneration
KW - bone morphogenetic protein-2
KW - guided bone regeneration
KW - nonsteroidal anti-inflammatory drugs
KW - salicylic acid
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U2 - https://doi.org/10.1177/0883911515603991
DO - https://doi.org/10.1177/0883911515603991
M3 - Article
VL - 31
SP - 140
EP - 151
JO - Journal of Bioactive and Compatible Polymers
JF - Journal of Bioactive and Compatible Polymers
SN - 0883-9115
IS - 2
ER -