VASE-encoded peptide modifies NCAM-and L1-mediated neurite outgrowth

Fritz Lahrtz, Rüdiger Horstkorte, Harold Cremer, Melitta Schachner, Dirk Montag

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

Interactions between the neural cell adhesion molecule (NCAM) with NCAM- expressing neurons (transinteraction) stimulate outgrowth of neurites. The extent of NCAM-triggered neurite outgrowth depends on the presence of 10 amino acids derived from the variable alternatively spliced exon (VASE or π- exon) in the fourth immunoglobulin-like domain of NCAM (Ig4): NCAM with VASE reduces and without VASE enhances neurite outgrowth in cis- or trans- interaction. We have investigated the role of VASE in neurite outgrowth by characterizing the receptors at the cell surface of cultured cerebellar neurons. Results from experiments with L1 and NCAM antibodies and with cerebellar neurons derived from wild-type or NCAM-deficient mice show that substrate-coated Ig4 with VASE (Ig4+) or without VASE (Ig4-) stimulates neurite outgrowth by a trans-interaction with L1 and that Ig4- promotes neurite outgrowth more strongly than Ig4+ by a transinteraction with NCAM.

Original languageEnglish (US)
Pages (from-to)62-68
Number of pages7
JournalJournal of Neuroscience Research
Volume50
Issue number1
DOIs
StatePublished - Oct 1 1997
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Cellular and Molecular Neuroscience

Keywords

  • L1
  • NCAM
  • Neurite outgrowth
  • VASE
  • π-exon

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