Yeast tRNAPhe conformation wheels: A novel probe of the monoclinic and orthorhombic models

A. R. Srinivasan, W. K. Olson

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

A series of conformation wheels is constructed from the recently refined X-ray crystallographic data of monoclinic and orthorhombic yeast tRNAPhe. These circular plots relate the primary chemical structure (i.e., base sequence) directly to the secondary and tertiary structure of the molecule. The circular sequence of backbone torsion angles displays a unique pattern that is useful both in distinguishing the ordered and disordered regions of the molecule and in comparing the three sets of experimental data. Composite conformation wheels describe the fluctuations in the "fixed" parameters (φ′, φ, χ) and independent conformation wheels reveal the changes in the "variable" parameters (ω′, ω, ψ, ψ′) of the three different yeast tRNAPhe models. Additional plots of base-stacking parameters help to visualize the intimate interrelationship between chemical sequence and three-dimensinnal folding of yeast tRNAPhe. The composite data illustrate several conformational schemes that position the bases of adjacent nucleosides in a parallel stacked array and reveal an even larger number of conformations that introduce bends or turns in the polynucleotide chain.

Original languageEnglish (US)
Pages (from-to)2307-2330
Number of pages24
JournalNucleic acids research
Volume8
Issue number10
DOIs
StatePublished - May 24 1980

All Science Journal Classification (ASJC) codes

  • Genetics

Fingerprint

Dive into the research topics of 'Yeast tRNA<sup>Phe</sup> conformation wheels: A novel probe of the monoclinic and orthorhombic models'. Together they form a unique fingerprint.

Cite this