Zeon-1, a member of a new maize retrotransposon family

Weiming Hu, O. Prem Das, Joachim Messing

Research output: Contribution to journalArticlepeer-review

54 Scopus citations

Abstract

We have previously shown that the tandemly duplicated 27 kDa maize storage protein locus underwent mitotic rearrangement to yield a single-copy allele in isolates of the inbred line A188. This rearrangement contains a new LTR retrotransposon, designated Zeon-1. This middle repetitive element of 7313 by had two long terminal repeats, a primer binding site, a polypurine tract and a gag-related open reading frame of 375 amino acids. Transcripts of the gag-related region were detected by the polymerase chain reaction (PCR) in certain maize tissues, and Western blots detected the gag-related protein in the same tissues. Moreover, the product of this mitotic rearrangement was shown to contain the same insertion site and 3′ LTR as Zeon-1, suggesting that this rearrangement occurs with unusual precision. Zeon elements were found to be present in teosinte and not present in the Gramineae wheat, barley, sorghum and rye.

Original languageEnglish (US)
Pages (from-to)471-480
Number of pages10
JournalMGG Molecular & General Genetics
Volume248
Issue number4
DOIs
StatePublished - Aug 1995

All Science Journal Classification (ASJC) codes

  • Genetics

Keywords

  • DNA rearrangement
  • LTR retrotransposon
  • Maize
  • Repetitive DNA sequences

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