Zinc-mediated helix capping in a triple-helical protein

Jie Liu, Jiayin Dai, Min Lu

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Specific sequence signals at α-helix termini can assist protein folding by punctuating and cueing secondary structural elements in the final native conformation. Here we report the crystallization of a 56-residue alanine-containing peptide, denoted Ala-10 56 , in the presence of Zn 2+ . The 1.7 Å crystal structure shows that Ala-10 56 forms a parallel trimeric coiled coil with three zinc ions anchoring distinct capping conformations at the amino-terminal ends of the three helices. In each polypeptide chain, the free α-amino nitrogen and carbonyl oxygen of the amino-terminal Ser residue coordinate to a Zn 2+ ion to form a five-membered chelate, and the syn-unidentate interaction of the Asp7 side chain with the Zn 2+ cation leads to the formation of a unique docking arrangement for helix capping. Moreover, the coordination of the zinc ion involves a neighboring trimer molecule in the crystal. Consequently, the crystal contacts are stabilized by carboxylate-Zn 2+ interactions between four Ala-1056 trimers in the crystal lattice. The observed synergy between the protein-zinc ion recognition and the helix-packing arrangements would contribute to the conformational specificity of the Ala-10 56 trimer.

Original languageEnglish (US)
Pages (from-to)5657-5664
Number of pages8
JournalBiochemistry
Volume42
Issue number19
DOIs
StatePublished - May 20 2003
Externally publishedYes

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Zinc
Ions
Conformations
Proteins
Protein folding
Peptides
Crystals
Protein Folding
Protein Sorting Signals
Crystallization
Crystal lattices
Alanine
Cations
Nitrogen
Crystal structure
Oxygen
Molecules

All Science Journal Classification (ASJC) codes

  • Biochemistry

Cite this

Liu, Jie ; Dai, Jiayin ; Lu, Min. / Zinc-mediated helix capping in a triple-helical protein. In: Biochemistry. 2003 ; Vol. 42, No. 19. pp. 5657-5664.
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Zinc-mediated helix capping in a triple-helical protein. / Liu, Jie; Dai, Jiayin; Lu, Min.

In: Biochemistry, Vol. 42, No. 19, 20.05.2003, p. 5657-5664.

Research output: Contribution to journalArticle

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